Four years after the events on Isla Nublar, a wealthy British family docks their yacht and has come ashore on nearby Isla Sorna, unaware that the island contains genetically engineered dinosaurs. Their daughter wanders off and is attacked by a group of Compsognathus, but is saved by her father and yacht crew.
An ailing Dr. John Hammond invites
mathematician Dr. Ian Malcolm to his residence to discuss the recent
incident. Hammond's company InGen, which created the dinosaurs, is now
headed by Hammond's nephew Peter Ludlow, who used the incident to take control
of the company from Hammond. Ian learns that InGen originally cloned the
dinosaurs on Isla Sorna, but the island was abandoned during a hurricane and
the animals were released into the wild to fend for themselves. Ludlow wants to
exploit the island's creatures to save InGen from bankruptcy. Hammond asks Ian
to join a team who will document the dinosaurs in their natural habitat, in
order to encourage a policy of non-interference. Ian's girlfriend, paleontologist Dr.
Sarah Harding, was hired and is already on Isla Sorna. Upon hearing this, Ian
reluctantly agrees to go to the island, but only to retrieve Sarah.
Ian travels there with Eddie
Carr, an equipment specialist and engineer, and Nick Van Owen, a video
documentarian and activist. They find Sarah amidst a herd of Stegosaurus,
but she is insistent on staying to continue her research. Ian is shocked when
he discovers that his daughter Kelly has stowed away with the team in a
trailer, which serves as their mobile base. Ludlow and a mercenary team arrive
on the island to capture dinosaurs, with help from big-game hunters Roland
Tembo and Ajay Sidhu, Roland's second-in-command Dieter Stark, and
paleontologist Dr. Robert Burke.
Malcolm's group realizes that
Ludlow and the InGen team are planning to take the captured specimens back to
the mainland and to an unfinished Jurassic Park attraction in San Diego,
an amphitheater zoo which Hammond abandoned in favor of the park on Isla
Nublar. Nick and Sarah free the captured dinosaurs, allowing the animals to
wreak havoc across the InGen team's camp. On the way back to the trailer, Nick
rescues an injured Tyrannosaurus rex infant that Roland tried
to use as bait to hunt its male parent. Nick and Sarah treat the infant for a
broken leg, but the Tyrannosaurus adults arrive. They reclaim
their infant, destroy the trailer, and devour Eddie during his attempt to
rescue the group.
Ian, Sarah, Nick, and Kelly are
rescued by Ludlow's team, and are forced to work together with them after
learning that they've lost all their communicative equipment as well. They
elect to walk en masse to a distant abandoned InGen base to
call for help. During a break, Stark goes into the wilderness alone to relieve
himself and is killed by a group of Compsognathus. After the team
sets up a camp for the night, they are ambushed by the Tyrannosaurus adults:
Burke is devoured, and everyone else flees into a field of long grass
containing a pack of Velociraptors, where Ajay and the others are
all killed.
Ian, Sarah, Kelly, and Nick
manage to reach the InGen base, fight off three Velociraptors, and
successfully radio for helicopter extraction. After being rescued, Nick reveals
that he stole Roland's ammunition to prevent him from killing his intended
trophy, but while passing overhead, they notice that Roland sedated the
male Tyrannosaurus. As more InGen personnel arrive on the island to
secure the male and the infant, Roland declines a job offer at the San Diego
park from Ludlow, reflecting on Ajay's death and the morality of Ludlow's
scheme.
In San Diego, Ian and Sarah
attempt to meet with Ludlow in order to prevent him from unveiling the
attraction. Before they can do this, the ship carrying the male Tyrannosaurus suddenly
crashes into the docks. The crew is found dead, and the Tyrannosaurus is
accidentally released and begins rampaging through the city. Ian and Sarah
locate the infant at the attraction and retrieve it, using it to lure the male
back to the docks. Ludlow pursues the couple onto the ship, and tries to catch
the infant within the cargo hold, but he is cornered by the male, which breaks
his leg, incapacitating him before being mauled to death by the infant. Sarah
uses a tranquilizer gun to sedate the male, while Ian seals the cargo hold
doors.
In the aftermath, the dinosaurs
are shipped back to Isla Sorna accompanied by a naval escort, while Ian, Sarah
and Kelly watch Hammond's announcement that the American and Costa Rican
governments have declared the island a nature preserve in a televised
interview.

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